On Site eDNA Assessment of Species-at-Risk (SAR): Implications for Aquatic Resource Management

Session: 09. - Modeling, Detecting, and Managing Rarity

Steven Crookes, University of Guelph, [email protected]
Amanda Naaum, University of Guelph, [email protected]
Mario Thomas, University of Guelph, [email protected]
Mary Murdoch, Stantec Inc., [email protected]
Bob Hanner, University of Guelph, [email protected]
Jasmine Sollen, University of Guelph, [email protected]

Abstract

Aquatic species encounter a plenitude of external, and increasingly anthropogenic, pressures that threaten their extant distribution, population connectivity, repository of standing genetic variation and – ultimately – their very survival. Continuous monitoring of species-at-risk (SAR) is an essential component in driving forward conservation efforts. Traditional monitoring methods are often constrained by limited resources, availability of expert personnel, seasonal and ontogenetic ecology of targets, and time. Monitoring levels of environmental DNA (eDNA) in the water column, through the application of real-time quantitative Polymerase Chain Reactions (qPCR), represents a highly sensitive, target-specific and potentially cost-effective means to assay aquatic SAR. Until recently, eDNA assays were dependent upon methodological constraints that required significant handling and processing of samples, alongside PCR analysis, that incur both Types I (false positive) and II (false negative) error. In this seminar, a new model of aquatic eDNA processing and data production is presented, synthesizing previously separate stages into a unified on site workflow. Analytical results are displayed and disseminated via the cloud in real-time. The impact that real-time eDNA data acquisition will have on the field will be discussed. In conclusion, key issues purporting to the adoption of this novel platform to study design will also be addressed.

1. Keyword
conservation

2. Keyword
genetics

3. Keyword
biodiversity

4. Additional Keyword
Biomonitoring