Semi-automated method for detecting and counting cells of cyanobacterial colonies and filaments

Session: 37. - Harmful Algal Blooms (HABs) and their Toxicity: Remote Sensing and Modeling Approaches

Frances Buerkens, Fluid Imaging Technologies, [email protected]
Peggy Lehman, California Department of Fish & Wildlife , [email protected]
Harry Nelson, Fluid Imaging Technologies, [email protected]
Andrew Bramburger, Natural Resources Research Institute, University of Minnesota Duluth, [email protected]

Abstract

Harmful algae blooms are increasing in frequency and intensity. Public safety and conservation agencies demand a replicable and scalable method to rapidly detect and enumerate cells comprising cyanobacterial colonies and filaments. The FlowCam is a proven technology that identifies taxa to the genus level and provides an estimate of the abundance of individual cells. It combines digital imaging, flow cytometry, and microscopy to calculate the dimensions, biovolume and abundance of cells. The FlowCam Cyano leverages recent technological developments – a 633 nm laser – enabling the instrument to distinguish cyanobacteria from other algae in a water sample. The abundance of cells within colonies and filaments are counted using a simple Excel based formula, enabling monitoring agencies and researchers to rapidly enumerate cells in large sample volumes. The FlowCam system facilitates an accurate measurement of cell abundance for large folded colonies because the colonies flatten within the unique flow cell chamber. Here we present an overview of the technology along with HAB field data from freshwater systems that affect drinking water and recreational lakes across North America.

1. Keyword
harmful algal blooms

2. Keyword
monitoring

3. Keyword
management

4. Additional Keyword
flow cytometry

5. Additional Keyword
technological innovation

6. Additional Keyword
automation