Development and application of a low volume flow system for in vivo solution-state NMR
Session: Poster session
Maryam Tabatabaei Anaraki, University of Toronto, [email protected]
Rudy Dutta Majumdar, University of Toronto Scarborough, [email protected]
Nicole Wagner, Cornell University, [email protected]
Ronald Soong, University of Toronto Scarborough, [email protected]
Eric Reiner, Ministry of Environment and Climate Change, [email protected]
Satyendra Bhavsar, Ontario Ministry of Environment and Climate Change, [email protected]
Xavier Ortiz Almirall, Ministry of Environment and Climate Change, [email protected]
Daniel Lane, University of Toronto Scarborough, [email protected]
Myrna Simpson, University of Toronto Scarborough, [email protected]
Andre Simpson, University of Toronto Scarborough, [email protected]
Abstract
In vivo NMR has the potential to monitor metabolic flux in close to real time, essential for better understanding the mode-of-action of a toxic contaminants and deciphering complex interconnected response pathways impacted inside the organism. Thus, a novel continuous low-volume flow system, suitable for in vivo NMR metabolic studies is introduced that allows organisms to be kept alive indefinitely. The novel system also improves spectrometer locking, shimming, water suppression and requires only a few mL of liquid enabling the study of sample-limited environmental matrices or very expensive contaminant standards. The use of double pump design with a sump slurry pump return, allows algal food suspensions to be continually supplied with water without the need for filters eliminating the possibility of clogging and leaks. To evaluate the feasibility and applicability of the flow system, metabolite changes in 13C enriched Daphnia magna (water flea) over a 24-hour period are compared when feeding laboratory food vs. exposing them to a natural algal bloom sample. Clear metabolic changes are observed over a range of metabolites including, carbohydrates, lipids, amino acids and a nucleotide demonstrating in vivo NMR as a powerful tool to monitor environmental stress.
1. Keyword
biomonitoring
2. Keyword
zooplankton
4. Additional Keyword
In vivo NMR
5. Additional Keyword
Metabolomics
6. Additional Keyword
Flow System