Time-dependent Daphnia magna metabolomics and potential bioacumulation with PFOS

Session: Poster session

Tae-Yong Jeong, University of Toronto Scarborough, [email protected]
Myrna Simpson, University of Toronto Scarborough, [email protected]

Abstract

Perfluorooctanesulfonic acid (PFOS) is a hazardous compound with high stability and bioaccumulation potential in freshwater aquatic species. Previous studies from our group observed perturbations to the Daphnia metabolome after acute PFOS exposure with non-monotonic metabolite concentration dependency. To investigate PFOS sub-lethal toxicity further, we examined the time course regulation and dynamics of the Daphnia magna. We targeted and monitored 26 metabolites using liquid chromatography with tandem mass spectrometry (LC-MS/MS). Additionally, the acute and chronic bioaccumulation potential of PFOS was monitored in D. magna during different developmental stages.  Monitoring of the metabolome hourly  revealed significant changes in metabolite concentrations with time due to PFOS exposure. PFOS bioaccumulation in D. magna was much higher in younger Daphnids. Such results suggest a potential ecological risk of PFOS and that time-series monitoring is needed to understand the adverse impacts and bioaccumulation of aquatic pollutants.

1. Keyword
PFOs

2. Keyword
zooplankton

3. Keyword
ecosystem health

4. Additional Keyword
Daphnia metabolomics